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2 edition of Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents found in the catalog.

Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents

Johnson, Richard A.

Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents

by Johnson, Richard A.

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Published by Mississippi-Alabama Sea Grant Consortium in [Ocean Springs, Miss.] .
Written in English

    Subjects:
  • Enteroviruses.,
  • Estuarine pollution -- Mississippi.,
  • Estuarine sediments -- Mississippi.,
  • Sewage disposal in the ocean -- Mississippi.

  • Edition Notes

    Statementby Richard A. Johnson and R.D. Ellender.
    SeriesMASGP -- 82-033.
    ContributionsEllender, R. D., Mississippi-Alabama Sea Grant Consortium.
    The Physical Object
    Pagination60 p. :
    Number of Pages60
    ID Numbers
    Open LibraryOL16108759M

    Richard A. Johnson has written: 'Synopsis of agricultural legislation and proposed regulations' -- subject(s): Agricultural laws and legislation 'Elution of enteric viruses from Mississippi. The variability of environmental water samples impacts the allowance of one method to be universally ideal for all water types and volumes. Surface and reclaimed waters can be used for crop irrigation and may be referred to as non-traditional irrigation waters as these water types may be associated with a higher risk of microbial contamination compared to groundwater.

    This report describes a recently developed method for the quantitative detection of enteroviruses in estuarine sediments. The method involves the elution of enteroviruses adsorbed to sediment with a solution of M ethylenediaminetetraacetate (pH ). The volume of the eluate is then reduced by membrane filter adsorption-elution into an economically assayable volume (30 to 50 ml). Human Enteric Viruses. Human enteric viruses are increasingly recognized as important causes of foodborne illness. A recent report issued by the Council for Agricultural Science and Technology ranked human enteric viruses as fifth and sixth among identified causes of foodborne disease in the United States.A review of U.S. national surveillance data for showed that 14 (44%) of

    The characteristics of these enteric virus groups will be discussed. Viruses are recovered and concentrated from water by passage through a positively charged cartridge filter. Following virus elution from the cartridge filter with beef extract and concentration of the beef extract solution, viruses are usually assayed by cell culture. Since viruses are only likely to be present in water at low levels, very large samples of many litres may need to be collected. Testing water samples for enteric viruses requires a specialised concentration step prior to isolation and detection. Methods used with some success include adsorption-elution techniques, precipitation and ultrafiltration.


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Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents by Johnson, Richard A. Download PDF EPUB FB2

Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents. Gerba CP. Influence of pH, salinity, and organic matter on the adsorption of enteric viruses to estuarine sediment.

Appl Environ Microbiol. Jul; 38 (1)–Cited by: Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents (MASGP) [Richard A Johnson] on *FREE* shipping on qualifying offers. Isoelectric casein supplemented with lecithin was tested for its ability to recover enteric viruses from estuarine sediments of varied sand, silt, and clay composition.

Recoveries were higher when lecithin was incorporated into an eluent as compared with trials with only the casein solution. Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents. Johnson RA, Ellender RD, Tsai SC.

Isoelectric casein supplemented with lecithin was tested for its ability to recover enteric viruses from estuarine sediments of varied sand, silt, and clay by: Buy Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents by Johnson, Richard A (ISBN:) from Amazon's Book Store.

Everyday low prices and free delivery on eligible : Richard A Johnson. Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents. By R A Johnson, R D Ellender and S C Tsai.

Abstract. Isoelectric casein supplemented with lecithin was tested for its ability to recover enteric viruses from estuarine sediments of varied sand, silt, and clay composition. Recoveries were higher. Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents.

(PMID PMCID:PMC) Abstract Citations; Related Articles Isoelectric casein supplemented with lecithin was tested for its ability to recover enteric viruses from estuarine sediments of varied sand, silt, and clay composition.

ABSTRACTRiver and estuarine sediment is suggested to play an important role in transmission of microbesin the water environment.

However, although effective methods to recover bacteria fromsediment are available, preparation methods for viruses, especially. Select up to three search categories and corresponding keywords using the fields to the right.

Refer to the HelpHelp. Elution of enteric viruses from Mississippi estuarine sediments with lecithin-supplemented eluents. Appl. Environ. Microbiol. OpenUrl Abstract / FREE Full Text. Development of an Effective Method for Recovery of Viral Genomic RNA from Environmental Silty Sediments for Quantitative Molecular Detection䌤 Takayuki Miura,1,2* Yoshifumi Masago,1 Daisuke Sano,2 and Tatsuo Omura1 Department of Civil and Environmental Engineering, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 'Elution of enteric viruses from Mississippi sediments using lecithin-supplemented eluents' -- subject(s): Enteroviruses, Estuarine pollution, Estuarine sediments, Sewage disposal in the ocean.

Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Warriner, in Improving the Safety of Fresh Fruit and Vegetables, Enteric viruses. Enteric viruses only reproduce within the human host and all follow the fecal–oral route of transmission (Cliver, ).The most significant characteristic of enteric viruses is the ease by which they can be transferred from person to person and the low infectious dose.

Pathogenic enteric viruses can be retained in municipal sewage sludge as has been reported by many researchers. Although the RT-PCR technique has been extensively employed for the virus detection from various environmental samples, the application of RT-PCR to the detection of viruses in sewage sludge has the difficulty because of inhibitory substances to the gene amplification.

enteric virus infections through shellfish grown in contaminated waters, con-taminated drinking water,food crops grown in land irrigated with wastewater and/or fertilized with sewage, and, to a lesser extent, sewage-polluted recre-ational waters (Tables and ).

Studies have documented the presence of enteric viruses in raw and. Recent efforts have also been made to use molecular methods for enteric virus detection in sediment (Miura et al.

; Staggemeier et al. a, b). Nonetheless, these methods often lack thorough. Hepatitis A and E viruses, 4. Concentration of enteric viruses, Adsorption/elution, Gauze pads, Electronegative membranes and cartridges, Electropositive cartridges and membranes, Concentration using glass powder, Concentration using glass wool, Secondary concentration, The long term survival of fecal indicator organisms (FIOs) and human pathogenic microorganisms in sediments is important from a water quality, human health and ecological perspective.

Typically, both bacteria and viruses strongly associate with particulate matter present in freshwater, estuarine and marine environments. This association tends to be stronger in finer textured sediments and is. A variety of enteric viruses have been examined for the effects of chilled temperature on their survival in a range of food matrices (reviewed by Baert et al.

) (Table ). Most of the studies found that enteric viruses remained infectious for periods exceeding the shelf-life of products (Table ). Nine approaches to recover viral RNA from environmental silty sediments were newly developed and compared to quantify RNA viruses in sediments using molecular methods.

Four of the nine approaches employed direct procedures for extracting RNA from sediments (direct methods), and the remaining five approaches used indirect methods wherein viral particles were recovered before RNA extraction.Human Pathogenic Viruses in the Marine Environment Figure Routes of transmission of enteric viruses to become contaminants of the marine environment.

this chapter the role of fecal–oral transmission is unknown. In spite that several coronaviruses are spread by this route, there is no current evidence that this mode of transmission.Aim: To determine the occurrence of eight human enteric viruses in surface water and sewage samples from different geographical areas in Kenya.

Methods and Results: Enteric viruses were recovered from the water and sewage sources by glass‐wool adsorption elution and/or polyethylene glycol/NaCl precipitation and detected by singleplex real‐time and conventional PCR and reverse transcriptase.